HEPES 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic Acid CAS 7365-45-9 99% Buffer Biological Buffer for Cell Culture
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Biological buffer is a weak acid or base substance, which can be used to maintain the acidity or alkalinity of a solution at the desired pH value after the addition of an acid or base, stop the solution's pH from undergoing rapid changes. There are a wide variety of biological buffers with good solubility, both acidic and alkaline. As pH regulator, it can be used in various fields including agriculture, food processing, biochemistry, pharmaceuticals, photography and so on. When applied in personal care products it can reduce the irritation of compounded products, strip the skin cuticle , keep a soft skin feeling and improve skin performance.
Product Name: HEPES CAS No.: 7365-45-9 Synonyms: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid; N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid Molecular Formula: C8H18N2O4S Molecular Weight: 238.30 EINECS: 230-907-9 Appearance: White crystalline powder Purity: ≥99.0% pH (1M solution): 5.0--6.5 pKa (25°C): 7.48--7.5 Melting Point: 234--238°C Solubility: Soluble in water (1 M at 20°C, clear) Applications: Biological buffer for cell culture, molecular biology, and biochemistry research RNA/DNA extraction, hybridization buffer, prehybridization buffer ELISA, protein purification, and enzyme assays Pharmaceutical intermediates and diagnostic reagents Storage: Store in a cool, dry place at 15--30°C; keep sealed Shelf Life: 24 months under proper storage




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