MES monohydrate, 145224-94-8, MES.H2O, 2-(N-Morpholino)ethanesulfonic acid monohydrate, biological buffer, Good's buffer, cell culture buffer, biochemical reagent, white powder, high purity 99.5%, pharmaceutical intermediate
Product Name: MES Monohydrate (MES.H2O) CAS No.: 145224-94-8 Synonyms: 2-(N-Morpholino)ethanesulfonic acid monohydrate, 4-Morpholineethanesulfonic acid monohydrate MF: C6H15NO5S MW: 213.25 EINECS: 224-632-3 MDL No.: MFCD00149409 Appearance: White Crystalline Powder Purity: ≥99.5% (Biochemical Grade / Pharma Grade) pKa: 6.1 (25°C) Buffer Range: pH 5.5--6.7 Solubility: 80 g/L (20°C, water), clear solution pH (10 g/L): 3.5--4.1 Melting Point: >300°C (decompose) Moisture: 7.0--9.0% (Karl Fischer) Heavy Metals: ≤5 ppm Storage: Room temperature, sealed, dry HS Code: 29349990
MES Monohydrate (CAS 145224-94-8) is a classic Good's buffer with a pKa of 6.1 at 25°C, providing stable pH buffering at 5.5--6.7. It is highly soluble in water, low UV absorption (A260≤0.04, A280≤0.02), and widely used in biochemistry, molecular biology, and pharmaceutical research. As a zwitterionic buffer, MES Monohydrate maintains pH stability in cell culture, protein purification, enzyme assays, and electrophoresis. It is non-toxic, low-irritant, and compatible with most biological systems, making it a preferred choice for lab and industrial applications.
Biological Buffer: Cell culture, tissue engineering, microbial culture Protein & Enzyme Research: Protein purification, enzyme activity assays, electrophoresis (small protein separation) Molecular Biology: DNA/RNA extraction, PCR buffer component Pharmaceutical: Biopharmaceutical formulation, diagnostic reagents Plant Cell Culture: Buffer for plant tissue culture and growth promotion